Review



capturem his tagged purification maxiprep kit  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    TaKaRa capturem his tagged purification maxiprep kit
    Capturem His Tagged Purification Maxiprep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/capturem+his+tagged+purification+maxiprep+kit/Capturem+His-Tagged+Purification+Maxiprep+Kit/pm42115618-697-19-24
    Average 99 stars, based on 46 article reviews
    capturem his tagged purification maxiprep kit - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Protein Purification:

    Article Title: Gut-binding peptides as potential tools to reduce virus binding to honey bee gut surface proteins.
    Article Snippet: Top 10 E. coli competent cells (Thermo Fisher Scientific, Waltham, MA, USA) were used for the expression of fusion proteins. .. Protein purification was carried out using the Capturem His-Tagged Purification Maxiprep Kit (Clontech, Mountain View, CA, USA). .. Imidazole was filtered out from all protein samples using Amicon Ultra-0.5 mL centrifugal filters Ultracel-3K (Millipore Sigma, St. Louis, MO, USA).

    Article Title: Gut-binding peptides as potential tools to reduce virus binding to honey bee gut surface proteins
    Article Snippet: Top 10 E. coli competent cells (Thermo Fisher Scientific, Waltham, MA, USA) were used for the expression of fusion proteins. .. Protein purification was carried out using the Capturem His-Tagged Purification Maxiprep Kit (Clontech, Mountain View, CA, USA). .. Imidazole was filtered out from all protein samples using Amicon Ultra-0.5 mL centrifugal filters Ultracel-3K (Millipore Sigma, St. Louis, MO, USA).

    Purification:

    Article Title: Gut-binding peptides as potential tools to reduce virus binding to honey bee gut surface proteins.
    Article Snippet: Top 10 E. coli competent cells (Thermo Fisher Scientific, Waltham, MA, USA) were used for the expression of fusion proteins. .. Protein purification was carried out using the Capturem His-Tagged Purification Maxiprep Kit (Clontech, Mountain View, CA, USA). .. Imidazole was filtered out from all protein samples using Amicon Ultra-0.5 mL centrifugal filters Ultracel-3K (Millipore Sigma, St. Louis, MO, USA).

    Article Title: Gut-binding peptides as potential tools to reduce virus binding to honey bee gut surface proteins
    Article Snippet: Top 10 E. coli competent cells (Thermo Fisher Scientific, Waltham, MA, USA) were used for the expression of fusion proteins. .. Protein purification was carried out using the Capturem His-Tagged Purification Maxiprep Kit (Clontech, Mountain View, CA, USA). .. Imidazole was filtered out from all protein samples using Amicon Ultra-0.5 mL centrifugal filters Ultracel-3K (Millipore Sigma, St. Louis, MO, USA).

    Article Title: Egyptian Rousette IFN-ω Subtypes Elicit Distinct Antiviral Effects and Transcriptional Responses in Conspecific Cells
    Article Snippet: .. Recombinant IFNs were purified from clarified media using the Capturem His-tagged purification maxiprep kit (Clontech, Takara Bio) and buffer-exchanged into sterile PBS with a Vivaspin 2 protein concentration column (MWCO 10 kDa; GE Life Sciences). .. Proteins were characterized by Western blot for the 6x-His tag (anti-6x-His tag mouse mAb, Thermo Fisher), silver staining for purity (Pierce Silver Stain assay kit; Thermo Fisher), and quantified by Bradford assay (Biorad, Hercules, CA) and by 280 nm absorbance measured on a NanoDrop spectrophotometer.

    Article Title: The Egyptian Rousette Genome Reveals Unexpected Features of Bat Antiviral Immunity
    Article Snippet: .. Proteins were purified via a Capturem His-tagged purification maxiprep kit (Clontech, Takara Bio, Mountain View, CA) and dialyzed into sterile 1x PBS with a Vivaspin 2 protein concentration column (MWCO 10kDa; GE Life Sciences). .. Proteins were quantified by western blot for the 6x-His tag, and by 280nm absorbance measured on a NanoDrop spectrophotometer.

    Article Title: Development of an enzyme-linked immunosorbent assay for Getah virus infection in horses using recombinant E2 protein as an antigen.
    Article Snippet: Getah virus causes fever, skin eruptions, and limb edema in horses.. For a high-throughput and time-saving method for serodiagnosis, we explored immunogenic antigens of Getah virus, and established an enzyme-linked immunosorbent assay (ELISA) using a recombinant protein.. Western blot analysis using sera from infected horses showed strong reaction with viral antigens around 46 kDa corresponding to E1 or E2 glycoproteins.

    Article Title: Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12.
    Article Snippet: After 48 h, bacterial and mammalian cells were collected by centrifugation and lysed in xTractor Buffer (Takara, #635656). .. Lysates were clarified at 50000 × g for 30 min at 4 °C, and supernatants were purified using the Capturem His-Tagged Purification Maxiprep Kit (Takara, #635713). .. Buffer exchange and concentration were performed with Slide-A-Lyzer MINI Dialysis Devices (Thermo Fisher Scientific, #88401) against 50 mM tris (pH 7.5, Thermo Fisher Scientific, #15567027), 500 mM NaCl, 1% glycerol (Thermo Fisher Scientific, #17904), 0.1 % CHAPS (Sigma Aldrich, #C3023) and 0.5 mM DTT (Thermo Fisher Scientific, #R0861).

    Article Title: The Egyptian Rousette Genome Reveals Unexpected Features of Bat Antiviral Immunity
    Article Snippet: .. Capturem His-tagged purification maxiprep kit , Clontech, Takara Bio , Cat# 635713. .. Vivaspin 2 Protein Concentrators, MWCO 10000 , GE Life Sciences , Cat# 28932247.

    Recombinant:

    Article Title: Egyptian Rousette IFN-ω Subtypes Elicit Distinct Antiviral Effects and Transcriptional Responses in Conspecific Cells
    Article Snippet: .. Recombinant IFNs were purified from clarified media using the Capturem His-tagged purification maxiprep kit (Clontech, Takara Bio) and buffer-exchanged into sterile PBS with a Vivaspin 2 protein concentration column (MWCO 10 kDa; GE Life Sciences). .. Proteins were characterized by Western blot for the 6x-His tag (anti-6x-His tag mouse mAb, Thermo Fisher), silver staining for purity (Pierce Silver Stain assay kit; Thermo Fisher), and quantified by Bradford assay (Biorad, Hercules, CA) and by 280 nm absorbance measured on a NanoDrop spectrophotometer.

    Sterility:

    Article Title: Egyptian Rousette IFN-ω Subtypes Elicit Distinct Antiviral Effects and Transcriptional Responses in Conspecific Cells
    Article Snippet: .. Recombinant IFNs were purified from clarified media using the Capturem His-tagged purification maxiprep kit (Clontech, Takara Bio) and buffer-exchanged into sterile PBS with a Vivaspin 2 protein concentration column (MWCO 10 kDa; GE Life Sciences). .. Proteins were characterized by Western blot for the 6x-His tag (anti-6x-His tag mouse mAb, Thermo Fisher), silver staining for purity (Pierce Silver Stain assay kit; Thermo Fisher), and quantified by Bradford assay (Biorad, Hercules, CA) and by 280 nm absorbance measured on a NanoDrop spectrophotometer.

    Article Title: The Egyptian Rousette Genome Reveals Unexpected Features of Bat Antiviral Immunity
    Article Snippet: .. Proteins were purified via a Capturem His-tagged purification maxiprep kit (Clontech, Takara Bio, Mountain View, CA) and dialyzed into sterile 1x PBS with a Vivaspin 2 protein concentration column (MWCO 10kDa; GE Life Sciences). .. Proteins were quantified by western blot for the 6x-His tag, and by 280nm absorbance measured on a NanoDrop spectrophotometer.

    Protein Concentration:

    Article Title: Egyptian Rousette IFN-ω Subtypes Elicit Distinct Antiviral Effects and Transcriptional Responses in Conspecific Cells
    Article Snippet: .. Recombinant IFNs were purified from clarified media using the Capturem His-tagged purification maxiprep kit (Clontech, Takara Bio) and buffer-exchanged into sterile PBS with a Vivaspin 2 protein concentration column (MWCO 10 kDa; GE Life Sciences). .. Proteins were characterized by Western blot for the 6x-His tag (anti-6x-His tag mouse mAb, Thermo Fisher), silver staining for purity (Pierce Silver Stain assay kit; Thermo Fisher), and quantified by Bradford assay (Biorad, Hercules, CA) and by 280 nm absorbance measured on a NanoDrop spectrophotometer.

    Article Title: The Egyptian Rousette Genome Reveals Unexpected Features of Bat Antiviral Immunity
    Article Snippet: .. Proteins were purified via a Capturem His-tagged purification maxiprep kit (Clontech, Takara Bio, Mountain View, CA) and dialyzed into sterile 1x PBS with a Vivaspin 2 protein concentration column (MWCO 10kDa; GE Life Sciences). .. Proteins were quantified by western blot for the 6x-His tag, and by 280nm absorbance measured on a NanoDrop spectrophotometer.

    Protein Extraction:

    Article Title: Development of an enzyme-linked immunosorbent assay for Getah virus infection in horses using recombinant E2 protein as an antigen.
    Article Snippet: Getah virus causes fever, skin eruptions, and limb edema in horses.. For a high-throughput and time-saving method for serodiagnosis, we explored immunogenic antigens of Getah virus, and established an enzyme-linked immunosorbent assay (ELISA) using a recombinant protein.. Western blot analysis using sera from infected horses showed strong reaction with viral antigens around 46 kDa corresponding to E1 or E2 glycoproteins.



    Similar Products

    99
    TaKaRa capturem his tagged purification maxiprep kit
    Capturem His Tagged Purification Maxiprep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/capturem+his+tagged+purification+maxiprep+kit/Capturem+His-Tagged+Purification+Maxiprep+Kit/pm42115618-697-19-24
    Average 99 stars, based on 1 article reviews
    capturem his tagged purification maxiprep kit - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa capturemtm his tagged purification maxiprep kit
    Capturemtm His Tagged Purification Maxiprep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/capturem+his+tagged+purification+maxiprep+kit/Capturem+His-Tagged+Purification+Maxiprep+Kit/pm41714022-76-10-15
    Average 99 stars, based on 1 article reviews
    capturemtm his tagged purification maxiprep kit - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa his tag affinity column capture his tagged purification maxiprep kit 635713 takara bio japan
    His Tag Affinity Column Capture His Tagged Purification Maxiprep Kit 635713 Takara Bio Japan, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/capturem+his+tagged+purification+maxiprep+kit/Capturem+His-Tagged+Purification+Maxiprep+Kit/pm41714024-54-7-16
    Average 99 stars, based on 1 article reviews
    his tag affinity column capture his tagged purification maxiprep kit 635713 takara bio japan - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa purification maxiprep kit
    Purification Maxiprep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/capturem+his+tagged+purification+maxiprep+kit/Capturem+His-Tagged+Purification+Maxiprep+Kit/pm40006955-87-9-12
    Average 99 stars, based on 1 article reviews
    purification maxiprep kit - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa capturem
    Capturem, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/capturem+his+tagged+purification+maxiprep+kit/Capturem+His-Tagged+Purification+Maxiprep+Kit/pmc11860666-96-7-12
    Average 99 stars, based on 1 article reviews
    capturem - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    TaKaRa purification kit
    Purification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/capturem+his+tagged+purification+maxiprep+kit/Capturem+His-Tagged+Purification+Maxiprep+Columns/bio_rxiv__2025__01__11__632565-265-11-14
    Average 99 stars, based on 1 article reviews
    purification kit - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results